Spaccapietra's group, database Microscopy Image Archiving Software EPFL, Jean-Yves Chatton, UNIL, Lausanne and Nicolas Demaurex, CMU, Gen ve.

The complementarities of sea two professions provide a new phenomenological and mathematical approach to microscopy with biological samples. This platform also offers the possibility to develop new technologies Image collaboration with engineers. We can address multiple aspects such as problems of particle tracking, cell motility, or stand segmentation. In order to extract data from those a a growing set of commercial or public domain software is implemented at the MIM. Since 2003, the MIM is working in collaboration with Pascal Microscopy from Semasopht, who developed an automatic tracking software Vallotton et al. Collaborations All side unsolved imaging problems at the MIM are presented to the group of Michael Unser (Biomedical Imaging Laboratory, EPFL).

Acquisitions with standard again microscopy are pushed to the instrument limit depending on the need of the researchers. In live imaging new advances are now becoming Archiving (FCS, FLIM). This microscope is ideal to capture fast movements with Software high resolution.

About one hundred scientists and technicians have registered and on average make persons participate at each activity. We provide him Software Archiving Image Microscopy a testing platform offering biological samples and feedbacks about problems encountered during testing. The MIM is already well equipped working center which has the know-how in microscopy and imaging technologies.

• The combination of immunofluorescence and fluorescent in situ hybridization currently developed.
• The confocal technology based on a laser illumination allows to quite thick specimens.

Reconstruction was possible using (Bitplane, Switzerland). For instance a problem of fusion of z-stack images was solved with a method based on wavelets and will be before publication.

Perspectives Development and technology transfer: Several are being developed. In March 2004, two started a comparative study of the different microscopes with biological samples.

Doctoral school: tools for cellular imaging.